Composite

Part:BBa_K3237020:Design

Designed by: Aubrey Demchuk, Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-14)


His-mRFP1-Proinsulin-CPP with furin cut sites codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 649
    Illegal AgeI site found at 761
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon optimized for E.coli . our project concerns itself with expressing insulin in micro algae. therefore, this construct was made for comparative purposes.

Source

This part is based on Tsinghua2014 and the mRFp1 gene in the registry BBa_E1010. The part was synthesized by TWIST.


References

1. Liang, J., and Yang, V. (2005) Insulin-cell penetrating peptide hybrids with improved intestinal absorption efficiency. Communications. 335, 734-738.

2. Hay, C., and Docherty, K. (2003) Enhanced expression of a furin-cleavable proinsulin. Journal of molecular endocrinology. 3, 597-607.